東京大学大学院新領域創成科学研究科先端生命科学専攻生命応答システム分野

研究業績

原著論文

2024年

Application of unimodal probability distribution models for morphological phenotyping of budding yeast.

.Ohya Y*, Ghanegolmohammadi F, Itto-Nakama K.
FEMS Yeast Res.
2024 Jan 9;24:foad056. doi: 10.1093/femsyr/foad056.

2023年

Prediction of ethanol fermentation under stressed conditions using yeast morphological data.

Itto-Nakama K, Watanabe S, Ohnuki S, Kondo N, Kikuchi R, Nakamura T, Ogasawara W, Kasahara K, Ohya Y*.
J Biosci Bioeng.
2023 Mar;135(3):210-216. doi: 10.1016/j.jbiosc.2022.12.008.

Targeted mutations produce divergent characteristics in pedigreed sake yeast strains.

Klinkaewboonwong N, Ohnuki S, Chadani T, Nishida I, Ushiyama Y, Tomiyama S, Isogai A, Goshima T, Ghanegolmohammadi F, Nishi N, Kitamoto K, Akao T, Hirata D*, and Ohya Y*.
Microorganisms.
2023, 11(5), 1274. doi.org/10.3390/microorganisms11051274

Is AI essential? Examining the need for deep learning in image-activated sorting of Saccharomyces cerevisiae.

Hayashi M, Ohnuki S, Tsai Y, Kondo N, Zhou Y, Zhang H, Ishii NT, Ding T, Herbig M, Isozaki A*, Ohya Y*, Goda K*.
Lab Chip.
2023 Sep 26;23(19):4232-4244. doi: 10.1039/d3lc00556a.

2022年

Jerveratrum-type steroidal alkaloids inhibit β-1,6-glucan biosynthesis in fungal cell walls.

Kubo K, Itto-Nakama K*, Ohnuki S, Yashiroda Y, Li SC, Kimura H, Kawamura Y, Shimamoto Y, Tominaga KI, Yamanaka D, Adachi Y, Takashima S, Noda Y, Boone C, Ohya Y*.
Microbiol Spectr.
2022 Jan 12;10(1):e0087321. doi: 10.1128/spectrum.00873-21.

High-throughput platform for yeast morphological profiling predicts the targets of bioactive compounds.

Ohnuki S#, Ogawa I#, Itto-Nakama K, Lu F, Ranjan A, Kabbage M, Gebre AA, Yamashita M, Li SC, Yashiroda Y, Yoshida S, Usui T, Piotrowski JS, Andrews B, Boone C, Brown GW, Ralph J, Ohya Y*.
NPJ Syst Biol Appl.
2022 Jan 27;8(1):3. doi: 10.1038/s41540-022-00212-1.

Assignment of unimodal probability distribution models for quantitative morphological phenotyping.

Ghanegolmohammadi F, Ohnuki S, Ohya Y*.
BMC Biol.
2022 Mar 31;20:81. doi: 10.1186/s12915-022-01283-6.

S-adenosyl-L-homocysteine extends lifespan through methionine restriction effects.

Ogawa T, Masumura K, Kohara Y, Kanai M, Soga T, Ohya Y, Blackwell TK, Mizunuma M.
Aging Cell
2022 Apr 7;e13604. doi: 10.1111/acel.13604.

Deep imaging flow cytometry.

Huang K, Matsumura H, Zhao Y, Herbig M, Yuan D, Mineharu Y, Harmon J, Findinier J, Yamagishi M, Ohnuki S, Nitta N, Grossman AR, Ohya Y, Mikami H, Isozaki A, Goda K.
Lab Chip.
2022 Mar 1;22(5):876-889. doi: 10.1039/d1lc01043c.

Intelligent sort-timing prediction for image-activated cell sorting.

Zhao Y, Isozaki A, Herbig M, Hayashi M, Hiramatsu K, Yamazaki S, Kondo N, Ohnuki S, Ohya Y, Nitta N, and Goda K.
Cytometry Part A
2022 June 29. doi: 10.1002/cyto.a.24664.

Multifarious Translational Regulation during Replicative Aging in Yeast .

Zhao T#, Chida A#, Shichino Y#, Choi D, Mizunuma M, Iwasaki S, Ohya Y*.
J Fungi (Basel)
2022 Sep 5; 8(9), 938. doi.org/10.3390/jof8090938.

BIONIC: biological network integration using convolutions.

Forster DT, Li SC, Yashiroda Y, Yoshimura M, Li Z, Isuhuaylas LAV, Itto-Nakama K, Yamanaka D, Ohya Y, Osada H, Wang B, Bader GD, Boone C.
Nature Methods
2022 Oct 3. doi: 10.1038/s41592-022-01616-x.

2021年

Atg15 in Saccharomyces cerevisiae consists of two functionally distinct domains.

Hirata E, Shirai K, Kawaoka T, Sato K, Kodama F, Suzuki K.
Mol. Biol. Cell
2021 Apr 15;32(8):645-663. doi: 10.1091/mbc.E20-07-0500. Epub 2021 Feb 24.

Genome editing to generate Sake yeast strains with eight mutations that confer excellent brewing characteristics.

Chadani T, Ohnuki S, Isogai A, Goshima T, Kashima M, Ghanegolmohammadi F, Nishi T, Hirata D, Watanabe D, Kitamoto K, Akao T, Ohya Y*.
Cells
2021 May 24;10(6):1299. doi: 10.3390/cells10061299.

Poacic acid, a beta-1,3-glucan-binding antifungal agent, inhibits cell-wall remodeling and activates transcriptional responses regulated by the cell-wall integrity and high-osmolarity glycerol pathways in yeast.

Garcìa R#, Itto-Nakama K#, Rodrìguez-Peña JM#, Chen X, Sanz AB, de Lorenzo A, Pavón-Vergés M, Kubo K, Ohnuki S, Nombela C, Popolo L, Ohya Y*, Arroyo J*.
FASEB J.
2021 Sep;35(9):e21778. doi: 10.1096/fj.202100278R.

Defining functions of mannoproteins in Saccharomyces cerevisiae by high-dimensional morphological phenotyping.

Ghanegolmohammadi F, Okada H, Liu Y, Itto-Nakama K, Ohnuki S, Savchenko A, Bi E, Yoshida S, Ohya Y*.
J Fungi (Basel)
2021 Sep 17;7(9):769. doi: 10.3390/jof7090769.

Are droplets really suitable for single-cell analysis? A case study on yeast in droplets.

Nakagawa Y#, Ohnuki S#, Kondo N, Itto-Nakama K, Ghanegolmohammadi F, Isozaki A*, Ohya Y*, Goda K*
Lab Chip.
2021 Sep 28;21(19):3793-3803. doi: 10.1039/D1LC00469G

AI-based forecasting of ethanol fermentation using yeast morphological data.

Itto-Nakama K#, Watanabe S#, Kondo N#, Ohnuki S#, Kikuchi R, Nakamura T, Ogasawara W, Kasahara K*, Ohya Y*.
Biosci Biotechnol Biochem.
2021 Dec 22;86(1):125-134. doi: 10.1093/bbb/zbab188

2020年

Phase separation organizes the site of autophagosome formation.

Fujioka Y, Alam JM, Noshiro D, Mouri K, Ando T, Okada Y, May AI, Knorr RL, Suzuki K, Ohsumi Y, Noda NN.
Nature
2020 Feb;578(7794):301-305. doi: 10.1038/s41586-020-1977-6. Epub 2020 Feb 5.

Callose synthesis suppresses cell death induced by low-calcium conditions in leaves.

Shikanai Y, Yoshida R, Hirano T, Enomoto Y, Li B, Asada M, Yamagami M, Yamaguchi K, Shigenobu S, Tabata R, Sawa S, Okada H, Ohya Y, Kamiya T, Fujiwara T.
Plant Physiol.
2020 Feb 5. pii: pp.00784.2019. doi: 10.1104/pp.19.00784. [Epub ahead of print]

Virtual-freezing fluorescence imaging flow cytometry.

Mikami H, Kawaguchi M, Huang CJ, Matsumura H, Sugimura T, Huang K, Lei C, Ueno S, Miura T, Ito T, Nagasawa K, Maeno T, Watarai H, Yamagishi M, Uemura S, Ohnuki S, Ohya Y, Kurokawa H, Matsusaka S, Sun CW, Ozeki Y, Goda K.
Nat Commun.
2020 Mar 6;11(1):1162. doi: 10.1038/s41467-020-14929-2.

Genetic profiling of protein burden and nuclear export overload.

Kintaka R, Makanae K, Namba S, Kato H, Kito K, Ohnuki S, Ohya Y, Andrews BJ, Boone C, Moriya H.
Elife
2020 Nov 4;9:e54080. doi: 10.7554/eLife.54080.

Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments.

Isozaki A, Nakagawa Y, Loo MH, Shibata Y, Tanaka N, Setyaningrum DL, Park JW, Shirasaki Y, Mikami H, Huang D, Tsoi H, Riche CT, Ota T, Miwa H, Kanda Y, Ito T, Yamada K, Iwata O, Suzuki K, Ohnuki S, Ohya Y, Kato Y, Hasunuma T, Matsusaka S, Yamagishi M, Yazawa M, Uemura S, Nagasawa K, Watarai H, Di Carlo D, Goda K.
Sci Adv.
2020 May 29;6(22):eaba6712. doi: 10.1126/sciadv.aba6712. eCollection 2020 May.

Intelligent image-activated cell sorting 2.0.

Isozaki A, Mikami H, Tezuka H, Matsumura H, Huang K, Akamine M, Hiramatsu K, Iino T, Ito T, Karakawa H, Kasai Y, Li Y, Nakagawa Y, Ohnuki S, Ota T, Qian Y, Sakuma S, Sekiya T, Shirasaki Y, Suzuki N, Tayyabi E, Wakamiya T, Xu M, Yamagishi M, Yan H, Yu Q, Yan S, Yuan D, Zhang W, Zhao Y, Arai F, Campbell RE, Danelon C, Di Carlo D, Hiraki K, Hoshino Y, Hosokawa Y, Inaba M, Nakagawa A, Ohya Y, Oikawa M, Uemura S, Ozeki Y, Sugimura T, Nitta N, Goda K.
Lab Chip.
2020 Jun 30;20(13):2263-2273. doi: 10.1039/d0lc00080a.

Liquidity is a critical determinant for selective autophagy of protein condensates.

Yamasaki A, Alam JM, Noshiro D, Hirata E, Fujioka Y, Suzuki K, Ohsumi Y, Noda NN.
Mol Cell.
2020 Jan 20. pii: S1097-2765(19)30956-6. doi: 10.1016/j.molcel.2019.12.026. [Epub ahead of print]

The kinetic landscape and interplay of protein networks in cytokinesis.

Okada H, MacTaggart B, Ohya Y, Bi E.
iScience
2020 Dec 11;24(1):101917. doi: 10.1016/j.isci.2020.101917. eCollection 2021 Jan 22.

2019年

Genome editing to generate nonfoam-forming sake yeast strains.

Ohnuki S, Kashima M, Yamada T, Ghanegolmohammadi F, Zhou Y, Goshima T, Maruyama JI, Kitamoto K, Hirata D, Akao T, Ohya Y.
Biosci Biotechnol Biochem.
2019 Aug;83(8):1583-1593. doi: 10.1080/09168451.2019.1631146. Epub 2019 Jun 12.

Implications of maintenance of mother-bud neck size in diverse vital processes of Saccharomyces cerevisiae.

Kubo K, Okada H, Shimamoto T, Kimori Y, Mizunuma M, Bi E, Ohnuki S, Ohya Y.
Current Genetics
2019 Feb;65(1):253-267. doi: 10.1007/s00294-018-0872-2. Epub 2018 Jul 31.

Simulated microgravity triggers characteristic morphology and stress response in Saccharomyces cerevisiae.

Nemoto S, Ohnuki S, Abe F, Ohya Y.
Yeast
2019 Feb;36(2):85-97. doi: 10.1002/yea.3361. Epub 2018 Nov 7.

Cucurbitacin B exerts antiaging effects in yeast by regulating autophagy and oxidative stress.

Lin Y, Kotakeyama Y, Li J, Pan Y, Matsuura A, Ohya Y, Yoshida M, Xiang L, Qi J.
Oxid Med Cell Longev.
2019 Jun 2;2019:4517091. doi: 10.1155/2019/4517091. eCollection 2019.

2018年

High-dimensional single-cell phenotyping reveals extensive haploinsufficiency.

Ohnuki S, Ohya Y.
PLoS Biol.
2018 May 16; 16(5):e2005130

Genetic dissection of the signaling pathway required for the cell wall integrity checkpoint.

Sukegawa Y, Negishi T, Kikuchi Y, Ishii K, Imanari M, Ghanegolmohammadi F, Nogami S, Ohya Y.
Journal of Cell Science
2018 May 31 pii: jcs.219063

Global study of holistic morphological effectors in the budding yeast Saccharomyces cerevisiae.

Suzuki G, Wang Y, Kubo K, Hirata E, Ohnuki S, Ohya Y.
BMC Genomics
2018 Feb 20;19(1):149

The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion.

Sing TL, Hung MP, Ohnuki S, Suzuki G, San Luis BJ, McClain M, Unruh JR, Yu Z, Ou J, Marshall-Sheppard J, Huh WK, Costanzo M, Boone C, Ohya Y, Jaspersen SL, Brown GW.
Journal of Cell Biology
2018 Jul 2;217(7):2445-2462.

Carotenoid dynamics and lipid droplet containing astaxanthin in response to light in the green alga Haematococcus pluvialis.

Ota S, Morita A, Ohnuki S, Hirata A, Sekida S, Okuda K, Ohya Y, Kawano S.
Scientific Reports
2018 Apr 8(1):5617

Yeast species-specific, differential inhibition of β-1,3-glucan synthesis by poacic acid and caspofungin.

Lee KK, Kubo K, Abdelaziz JA, Cunningham I, da Silva Dantas A, Chen X, Okada H, Ohya Y, Gow NAR.
The Cell Surface
2018 Sept 26; 3:12-25.

Predicting bioprocess targets of chemical compounds through integration of chemical-genetic and genetic interactions.

Simpkins SW, Nelson J, Deshpande R, Li SC, Piotrowski JS, Wilson EH, Gebre AA, Safizadeh H, Okamoto R, Yoshimura M, Costanzo M, Yashiroda Y, Ohya Y, Osada H, Yoshida M, Boone C, Myers CL.
PLoS Comput Biol.
2018 Oct 30; 14(10):e1006532. doi: 10.1371/journal.pcbi.1006532.

2017年

Testing the neutral hypothesis of phenotypic evolution.

Ho WC, Ohya Y, Zhang J.
Proc Natl Acad Sci U S A
2017 Nov 14;114(46):12219-12224

Morphometric analysis of autophagy-related structures in Saccharomyces cerevisiae.

Kawaoka T, Ohnuki S, Ohya Y, Suzuki K.
Autophagy
2017;13(12):2104-2110

Atg4 plays an important role in efficient expansion of autophagic isolation membranes by cleaving lipidated Atg8 in Saccharomyces cerevisiae.

Hirata E, Ohya Y, Suzuki K.
PLOS One
2017 July 13. 12(7):e0181047

Systematic analysis of Ca²⁺ homoeostasis in Saccharomyces cerevisiae based on chemical-genetic interaction profiles.

Ghanegolmohammadi F, Yoshida M, Ohnuki S, Sukegawa Y, Okada H, Obara K, Kihara A, Suzuki K, Kojima T, Yachie N, Hirata D, Ohya Y.
Mol Cell Biol.
2017 Nov 7;28(23):3415-3427. doi: 10.1091/mbc.E17-04-0216. Epub 2017 May 31.

Phenotypic diagnosis of lineage and differentiation during Sake yeast breeding

Ohnuki S, Okada H, Friedrich A, Kanno Y, Goshima T, Hasuda H, Inahashi M, Okazaki N, Tamura H, Nakamura R, Hirata D, Fukuda H, Shimoi H, Kitamoto K, Watanabe D, Schacherer J, Akao T*, Ohya Y*.
G3: Genes, Genomes, Genetics
2017 Aug 7;7(8):2807-2820. doi: 10.1534/g3.117.044099.

Functional annotation of chemical libraries across diverse biological processes

Piotrowski JS, Li SC, Deshpande R, Simpkins SW, Nelson J, Yashiroda Y, Barber JM, Safizadeh H, Wilson E, Okada H, Gebre AA, Kubo K, Torres NP, LeBlanc MA, Andrusiak K, Okamoto R, Yoshimura M, DeRango-Adem E, van Leeuwen J, Shirahige K, Baryshnikova A, Brown GW, Hirano H, Costanzo M, Andrews B, Ohya Y, Osada H, Yoshida M, Myers CL, Boone C.
Nat Chem Biol.
2017 Jul 24. nchembio.2436

The dual activity responsible for the elongation and branching of β-(1,3)-glucan in the fungal cell wall.

Aimanianda V, Simenel C, Garnaud C, Clavaud C, Tada R, Barbin L, Mouyna I, Heddergott C, Popolo L, Ohya Y, Delepierre M, Latge JP
mBio
2017 Jun 20;8(3): e00619-17

Promoter engineering of the Saccharomyces cerevisiae RIM15 gene for improvement of alcoholic fermentation rates under stress conditions.

Watanabe D, Kaneko A, Sugimoto Y, Ohnuki S, Takagi H, Ohya Y.
J Biosci Bioeng.
2017 Feb;123(2):183-189.

2016年

The Late S-phase transcription factor Hcm1 is regulated through phosphorylation by the cell wall integrity checkpoint.

Negishi T, Veis J, Hollenstein D, Sekiya M, Ammerer G, and Ohya Y.
Mol Cell Biol.
2016 Jan 4;36(6):941-53. Epub 2016 Jan 4.

Zds1/Zds2-PP2ACdc55 complex specifies signaling output from Rho1 GTPase.

Jonasson EM, Rossio V, Hatakeyama R, Abe M, Ohya Y, and Yoshida S.
J Cell Biol.
2016 Jan 4;212(1):51-61.

Exploiting Single-Cell Quantitative Data to Map Genetic Variants Having Probabilistic Effects.

Chuffart F, Richard M, Jost D, Burny C, Duplus-Bottin H, Ohya Y, Yvert G.
PLoS Genet.
2016 Aug 1;12(8):e1006213.

Large-Scale Survey of Intraspecific Fitness and Cell Morphology Variation in a Protoploid Yeast Species.

Jung PP#, Sigwalt A#, Ohnuki S#, de Montigny J, Ohya Y, Schacherer J.
G3 (Bethesda).
2016 Apr 7;6(4):1063-71.

Identification of a mutation causing a defective spindle assembly checkpoint in high ethyl caproate-producing sake yeast strain K1801.

Goshima T, Nakamura R, Kume K, Okada H, Ichikawa E, Tamura H, Hasuda H, Inahashi M, Okazaki N, Akao T, Shimoi H, Mizunuma M, Ohya Y, Hirata D.
Biosci Biotechnol Biochem.
2016 Aug;80(8):1657-62. Epub 2016 May 18.

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN,Baryshnikova A, Pons C, Tan G,Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB,Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Mattiazzi Usaj M, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C.
Science
2016 Sep 23;353(6306).

Examination and disruption of the cell wall.

Okada H, Kono K, Neiman AM, and Ohya Y.
Cold Spring Harbor Protocols
Aug 1;2016(8):pdb.top078659.

Local and Acute Disruption of the Yeast Cell Surface.

Kono K, Okada H, Ohya Y.
Cold Spring Harb Protoc.
2016 Aug 1;2016(8):pdb.prot085266.

Assay for Spore Wall Integrity Using a Yeast Predator.

Okada H, Neiman AM, Ohya Y.
Cold Spring Harb Protoc.
2016 Aug 1;2016(8):pdb.prot085258.

Fluorescent Labeling of Yeast Cell Wall Components.

Okada H, Ohya Y.
Cold Spring Harb Protoc.
2016 Aug 1;2016(8):pdb.prot085241.

2015年

The plant derived, antifungal agent poacic acid targets β-1,3-glucan.

Piotrowski J#, Okada H#, Lu F, Li S, Hinchman L, Ranjan A, Smith D, Higbee A, Ulbrich A, Coon J, Deshpande R, Bukhman Y, McIlwain S, Ong I, Myers C, Boone C, Landick R, Ralph J, Kabbage M, and Ohya Y.
Proc Natl Acad Sci U S A.
2015 Mar 24;112(12):E1490-7. Epub 2015 Mar 9.

Casein kinase 1γ ensures monopolar growth polarity under incomplete DNA replication downstream of Cds1 and calcineurin in fission yeast.

Koyano T, Konishi M, Martin SG, Ohya Y, Hirata D, Toda T, and Kume K.
Mol Cell Biol.
2015 May;35(9):1533-42. Epub 2015 Feb 17.

Visualization of Atg3 during autophagosome formation in Saccharomyces cerevisiae.

Ngu MP, Hirata E, and Suzuki K.
J Biol Chem.
2015 Mar 27;290(13):8146-53. Epub 2015 Feb 2.

Single-cell phenomics in budding yeast.

Ohya Y, Kimori Y, Okada H, and Ohnuki S.
Mol Biol Cell.
2015 Nov 5;26(22):3920-5.

Isolation of a spontaneous cerulenin-resistant sake yeast with both high ethyl caproate-producing ability and normal checkpoint integrity

Tamura H*, Okada H, Kume K, Goshima T, Nakamura R, Akao T, Shimoi H, Mizunuma M, Ohya Y, and Hirata D*
Biosci. Biotechnol. Biochem.
2015;79(7):1191-9. Epub 2015 Mar 19.

Profiling of the effects of antifungal agents on yeast cells based on morphometric analysis.

Gebre AA, Okada H, Kim C, Kubo K, Ohnuki S, Ohya Y.
FEMS Yeast Res.
2015 Aug;15(5):fov040. Epub 2015 Jun 10.

Inhibitory role of Greatwall-like protein kinase Rim15p in alcoholic fermentation via upregulating the UDP-glucose synthesis pathway in Saccharomyces cerevisiae.

Watanabe D, Zhou Y, Hirata A, Sugimoto Y, Takagi K, Akao T, Ohya Y, Takagi H, and Shimoi H.
Appl Environ Microbiol.
2015 Oct 23;82(1):340-51.

Quantification of cell, actin and nuclear DNA morphology with high-throughput microscope and CalMorph.

Okada H, Ohnuki S, and Ohya Y.
Cold Spring Harbor Protocols
2015 Apr 1;2015(4):408-12.

Image-based prediction of drug target in yeast.

Ohnuki S, Okada H, and Ohya Y.
Methods Mol Biol.
2015;1263:319-27.

2014年

Vanillin causes the activation of Yap1 and mitochondrial fragmentation in Saccharomyces cerevisiae.

Nguyen TTM, Iwaki A, Ohya Y and Izawa S.
Journal of Bioscience and Bioengineering
2014 Jan;117(1):33-8. Epub 2013 Jul 11

Distinct roles of cell wall biogenesis in yeast morphogenesis as revealed by multivariate analysis of high-dimensional morphometric data.

Okada H, Ohnuki S, Roncero C, Konopka JB, Ohya Y.
Molecular Biology of the Cell
2014 Jan;25(2):222-33 Epub 2013 Nov 20

Proteomic profiling of autophagosome cargo in Saccharomyces cerevisiae.

Suzuki K, Nakamura S, Morimoto M, Fujii K, Noda NN, Inagaki F, Ohsumi Y.
PLoS One
2014 Mar 13;9(3):e91651.

Dynamic changes in brewing yeast cells in culture revealed by statistical analyses of yeast morphological data.

Ohnuki S, Enomoto K, Yoshimoto H and Ohya Y.
Journal of Bioscience and Bioengineering
2014 Mar;117(3):278-84. Epub 2013 Sep 6

Hyperspectral imaging techniques for the characterization of Haematococcus pluvialis (Chlorophyceae).

Nogami S, Ohnuki S and Ohya Y.
Journal of Phycology
2014 Oct;50(5):939-47. Epub 2014 Sep 17

Unveiling nonessential gene deletions that confer significant morphological phenotypes beyond natural yeast strains.

Yang M, Ohnuki S, Ohya Y.
BMC Genomics
2014 Oct 25;15:932

2013年

Vanillin Inhibits Translation and Induces Messenger Ribonucleoprotein (mRNP) Granule Formation in Saccharomyces cerevisiae: Application and Validation of High-Content, Image-Based Profiling.

Iwaki A, Ohnuki S, Suga Y, Izawa S and Ohya Y.
PLoS ONE
2013 Apr 24;8(4):e61748.

Single-cell phenomics reveals intra-species variation of phenotypic noise in yeast.

Yvert G, Ohnuki S, Nogami S, Imanaga Y, Fehrmann S, Schacherer J and Ohya Y.
BMC systems biology
2013 Jul 3;7(1):54.

Profilin is required for Ca²⁺ homeostasis and Ca²⁺-modulated bud formation in yeast.

Yoshida M, Ohnuki S, Yashiroda Y and Ohya Y.
Molecular Genetics and Genomics
2013 Aug;288(7-8):317-28. Epub 2013 May 26.

Organelle acidification is important for localization of vacuolar proteins in Saccharomyces cerevisiae.

Matsumoto R, Suzuki S and Ohya Y
Protoplasma
2013 Dec;250(6):1283-93. Epub 2013 May 25.

Image-Based Monitoring System for Green Algal Haematococcus pluvialis (Chlorophyceae) Cells during Culture.

Ohnuki S, Nogami S, Ota S, Watanabe K, Kawano S and Ohya Y.
Plant and Cell Physiology
2013 Nov;54(11):1917-29. Epub 2013 Sep 20.

2012年

Analysis of the biological activity of a novel 24-membered macrolide JBIR-19 in Saccharomyces cerevisiae by the morphological imaging program CalMorph.

Ohnuki S, Kobayashi T, Ogawa H, Kozone I, Ueda JY, Takagi M, Shin-Ya K, Hirata D, Nogami S and Ohya Y.
FEMS Yeast Research
2012 May;12(3):293-304. Epub 2012 Jan 3.

2011年

Ethanol fermentation driven by elevated expression of the G₁ cyclin gene CLN3 in sake yeast.

Watanabe D, Nogami S, Ohya Y, Kanno Y, Zhou Y, Akao T, Shimoi H.
Journal of Bioscience and Bioengineering
2011 Dec;112(6):577-82. Epub 2011 Sep 7.

2010年

Fission yeast GC kinase Ppk11 interacts with Pmo25 and plays an auxiliary role in concert with the morphogenesis Orb6 network (MOR) in cell morphogenesis.

Goshima T, Kume K, Koyano T, Ohya Y, Toda T, Hirata D.
The Journal of Biological Chemistry
2010 Nov 5;285(45):35196-205. Epub 2010 Sep 7.

Role of bottom-fermenting brewer's yeast KEX2 in high temperature resistance and poor proliferation at low temperatures

Yamagishi H, Ohnuki S, Nogami S, Ogata T and Ohya Y
The Journal of General and Applied Microbiology
Vol. 56 (2010) , No. 4 pp.297-312

Knr4 N-terminal domain controls its localization and function during sexual differentiation and vegetative growth.

Dagkessamanskaia A, Azzouzi KE, Kikuchi Y, Timmers T, Ohya Y, François JM and Martin YH
Yeast
2010 Aug;27(8):563-74.

High-content, image-based screening for drug targets in yeast

Ohnuki S, Oka S, Nogami S and Ohya Y
PLoS ONE
2010 Apr 14;5(4):e10177.

Multiple Functional Domains of the Yeast 1,3-β-Glucan Synthase Subunit Fks1p Revealed by Quantitative Phenotypic Analysis of Temperature-Sensitive Mutants

Okada H, Abe M, Asakawa-Minemura M, Hirata A, Qadota H, Morishita K, Ohnuki S, Nogami S, Ohya Y
Genetics
2010 Apr;184(4):1013-24. Epub 2010 Feb 1.

2009年

Comprehensive and quantitative analysis of yeast deletion mutants defective in apical and isotropic bud growth

Watanabe M, Watanabe D, Nogami S, Morishita S, Ohya Y
Current Genetics
2009 May 55(4);365-80. Epub 2009 May 24.

Multidimensional quantification of subcellular morphology of Saccharomyces cerevisiae using CalMorph, the high-throughput image-processing program

Negishi T, Nogami S, Ohya Y.
Journal of Biotechnology
2009 May 20;141(3-4):109-17. Epub 2009 Mar 31.

A microfluidic device to acquire high-magnification microphotographs of yeast cells

Ohnuki S, Nogami S, Ohya Y.
Cell Division
2009 Mar 24;4:5. Epub 2009 Mar 24.

Novel 24-membered macrolides, JBIR-19 and -20 isolated from Metarhizium sp. fE61

Kozone I, Ueda J, Watanabe M, Nogami S, Nagai A, Inaba S, Ohya Y, Takagi M, Shin-ya K
Journal of Antibiotics
2009 Mar;62(3):159-62. Epub 2009 Feb 6.

2008年

G1/S Cyclin-Dependent Kinase Regulates Small GTPase Rho1p through Phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae.

Kono K, Nogami S, Abe M, Nishizawa M, Morishita S, Pellman D, Ohya Y.
Mol Biol Cell
2008 Apr;19(4):1763-71. Epub 2008 Feb 6.

2007年

Conditional genomic rearrangement by designed meiotic recombination using VDE (PI-SceI) in yeast.

Fukuda T, Ohya Y, Ohta K.
Mol Genet Genomics
2007 Oct;278(4):467-78. Epub 2007 Jun 22.

Diversity of Ca²⁺-induced morphology revealed by morphological phenotyping of Ca²⁺-sensitive mutants of Saccharomyces cerevisiae.

Ohnuki S, Nogami S, Kanai H, Hirata D, Nakatani Y, Morishita S, Ohya Y.
Eukaryotic Cell
2007 May;6(5):817-30. Epub 2007 Mar 9.

Genetic complexity and QTL mapping of yeast morphological traits.

Nogami S, Ohya Y, Yvert G
PLOS Genet.
2007 Feb 23;3(2):e31

Involvement of small GTPase Rho1p in cell size control in yeast.

Kikuchi Y, Mizuuchi E, Nogami S, Morishita S, and Ohya Y.
FEMS Yeast Res.
2007 Jun;7(4):569-78. Epub 2007 Jan 16.

Homologous subunits of yeast 1,3-beta-glucan synthase are important for spore wall assembly in Saccharomyces cerevisiae.

Ishihara S, Hirata A, Nogami S, Beauvais A, Latge JP, Ohya Y.
Eukaryotic Cell
2007 Feb;6(2):143-56. Epub 2006 Dec 8.

2006年

Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis.

Yoshida S, Kono K, Lowery DM, Bartolini S, Yaffe MB, Ohya Y, Pellman D.
Science
2006 Jul 7;313(5783):108-11. Epub 2006 Jun 8.

Exploring the mode-of-action of bioactive compounds by chemical-genetic profiling in yeast.

Parsons AB, Lopez A, Givoni IE, Williams DE, Gray CA, Porter J, Chua G, Sopko R, Brost RL, Ho CH, Wang J, Ketela T, Brenner C, Brill JA, Fernandez GE, Lorenz TC, Payne GS, Ishihara S, Ohya Y, Andrews B, Hughes TR, Frey BJ, Graham TR, Andersen RJ, Boone C.
Cell
2006 Aug 11;126(3):611-25.

Investigation of the mechanism of meiotic DNA cleavage by VMA1-derived endonuclease uncovers a meiotic alteration in chromatin structure around the target site.

Fukuda T, Ohta K, Ohya Y.
Eukaryot Cell.
2006 Jun;5(6):981-90.

Evaluation of image processing programs for accurate measurement of budding and fission yeast morphology.

Suzuki, G., Sawai, H., Ohtani, M., Nogami, S., Sano-Kumagai, F., Saka, A., Yukawa, M., Saito, TL., Sese, J., Hirata, D,. Morishita, S., Ohya, Y.
Curr Genet.
2006 Apr;49(4):237-47. Epub 2006 Jan 6.

Recruitment of RecA homologs Dmc1p and Rad51p to the double-strand break repair site initiated by meiosis-specific endonuclease VDE (PI-SceI).

Fukuda T, Ohya Y.
Mol Genet Genomics.
2006 Feb;275(2):204-14. Epub 2005 Dec 9.

2005年

High-dimensional and large-scale phenotyping of yeast mutants.

Ohya, Y., Sese, J., Yukawa, M., Sano, F., Nakatani, Y., Saito, TL., Saka. A., Fukuda, T., Ishihara, S., Oka, S., Suzuki, G., Watanabe, M., Hirata, A., Ohtani, M., Sawai, H., Fraysse, N., Latge, JP., Francois, JM., Aebi, M., Tanaka, S., Muramatsu, S., Araki, H., Sonoike, K., Nogami, S., Morishita, S.
Proc Natl Acad Sci U S A.
2005 Dec 27;102(52):19015-20. Epub 2005 Dec 19.

Piperazine propanol derivative as a novel antifungal targeting 1,3-beta-D-glucan synthase.

Kondoh, O., Inagaki, Y., Fukuda, H., Mizuguchi, E., Ohya, Y., Arisawa, M., Shimma, N., Aoki, Y., Sakaitani, M., Watanabe, T.
Biol Pharm Bull.
2005 Nov;28(11):2138-41.

Data Mining Tools for Saccharomyces Cerevisiae Morphological Database.

Saito, T., Sese, J., Nakatani, Y., Sano F., Yukawa, M., Ohya, Y., and Morishita S.
Nuc. Acid Res.
2005 Jul 1;33(Web Server issue):W753-7.

Molecular dissection of ARP1 regions required for nuclear migration and cell wall integrity checkpoint functions in Saccharomyces cerevisiae.

Igarashi R, Suzuki M, Nogami S, Ohya Y.
Cell Struct Funct.
2005 30(2):57-67.

Involvement of actin and polarisome in morphological change during spore germination of Saccharomyces cerevisiae.

Kono, K., Matsunaga, R., Hirata, A., Suzuki, G., Abe, M., and Ohya, Y.
Yeast
2005 Jan 30;22(2):129-39.

Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs: Implications for the molecular pathogenesis of human mitochondrial diseases.

Umeda, N., Suzuki, T., Yukawa, M., Ohya, Y., Shindo, H., Watanabe, K., and Suzuki, T.
J. Biol. Chem.
2005 Jan 14;280(2):1613-24. Epub 2004 Oct 26.

2004年

Dynactin complex is involved in a checkpoint to monitor cell wall synthesis in Saccharomyces cerevisiae.

Suzuki, M., Igarashi, R., Sekiya, M., Utsugi, T., Morishita, S., Yukawa, M. and Ohya, Y.
Nat. Cell Biol.
2004 6: 861-71.

A novel mechanism of intragenic complementation between Phe to Ala calmodulin mutations.

Okano, H. and Ohya, Y.
J. Biochem.
2004 135:289-295.

Cell shape and growth of budding yeast cells in restrictive microenvironments.

Suzuki, M., Asada, Y., Watanabe, D., and Ohya Y.
Yeast
2004 21:983-9.

Molecular mechanism of vde-initiated intein homing in yeast nuclear genome.

Fukuda T, Nagai Y, Ohya Y.
Adv Biophys.
2004 38:215-232.

SCMD:Saccaromyces cerevisiae morphological database.

Saito, T.L., Ohtani, M., Sawai, H., Sano, F., Saka, A., Watanabe, D., Yukawa, M., Ohya, Y., and Morishita, S.
Nuc. Acid Res.
2004 32:D319-22.

Analysis of the molecular interaction of the farnesyl moiety of transducin through use of a photoreactive farnesyl analog.

Hagiwara, K., Wada, A., Katadae, M., Ito, M., Ohya, Y., Casey, P. J., and Fukada, Y.
Biochemstry
2004 43:300-309.

Development of Image Processing Program for Yeast Cell Morphology.

Ohtani, M., Sano, F., Saka, A., Ohya, Y., and Morishita, S.
J. Bioinfo. Comput. Biol.
2004 1:695-709.

2003年

Overexpression of S-adenosylmethionine decarboxylase (SAMDC) in Xenopus embryos activates maternal program of apoptosis as a "fail-safe" mechanism of early embryogenesis.

Kai, M., Kaito, C., Fukamachi, H., Higo, T., Takayama, E., Hara, H., Ohya, Y., Igarashi, K. and Shiokawa, K.
Cell Res.
2003 13:147-158.

Lack of GTP-bound Rho1p in secretory vesicles of Saccharomyces cerevisiae.

Abe, M., Qadota, H., Hirata, A. and Ohya, Y.
J. Cell. Biol.
2003 162: 85-97.

Binding of calmodulin to Nuf1p is required for karyogamy in Saccharomyces cerevisiae.

Okano, H. and Ohya, Y.
Mol. Genet. Genomics
2003 269:649-57

VDE-initiated intein homing in Saccharomyces cerevisiae proceeds in a meiotic recombination-like manner.

Fukuda, T., Nogami, S. and Ohya, Y.
Genes. Cells.
2003 8: 587-602.

Occurrence, horizontal transfer and degeneration of VDE intein family in Saccharomycete yeasts.

Okuda Y., Sasaki, D., Nogami, S., Kaneko, Y., Ohya, Y. and Anraku, Y.
Yeast
2003 20: 563-573.

Karyopherin-Mediated Nuclear Import of the Homing Endonuclease VMA1-Derived Endonuclease Is Required for Self-Propagation of the Coding Region.

Nagai, Y, Nogami, S., Kumagai-Sano, F. and Ohya, Y.
Mol. Cell. Biol.
2003 23: 1726-1736.

2002年

Movement of yeast 1,3-β-glucan synthase is essential for uniform cell wall synthesis.

Utsugi, T., Minemura, M., Hirata, A., Abe, M., Watanabe, D. and Ohya, Y.
Genes. Cells
2002 7: 1-9.

Protein-splicing reaction via a thiazolidine intermediate: crystal structure of the VMA1-derived endonuclease bearing the N and C-terminal propeptides.

Mizutani, R., Nogami, S., Kawasaki, M., Ohya, Y., Anraku, Y. and Satow, Y.
J. Mol. Biol.
2002 316: 919-929.

Mutations in Fks1p affect the cell wall content of β-1,3- and β-1,6-glucan in Saccharomyces cerevisiae.

Dijkgraaf, G. J. P., Abe, M., Ohya, Y. and Bussey, H.
Yeast
2002 19: 671-690.

Homing at an Extragenic Locus Mediated by VDE (PI-SceI) of Saccharomyces cerevisiae.

Nogami, S., Fukuda, T., Nagai, Y., Yabe, S., Sugiura, M., Mizutani, R., Satow, Y., Anraku, Y. and Ohya, Y.
Yeast
2002 19: 773-782.

Dissection of Upstream Regulatory Components of the Rho1p Effector, 1,3-β-Glucan Synthase, in Saccharomyces cerevisiae.

Sekiya-K., M., Abe, M., Saka, A., Watanabe, D., Kono, K., Minemura-A., Ishihara, S., M., Watanabe, T. and Ohya, Y.
Genetics
2002 162: 663-676.

2001年

A role for the Pkc1p/Mpk1p kinase cascade in the morphogenesis checkpoint.

Harrison, J. C., Bardes, E. S. G., Ohya, Y. and Lew, D. J.
Nat. Cell. Biol.
2001 3: 417-420.

A mutation in SPC42, which encodes a component of the spindle pole body, results in production of two-spored asci in Saccharomyces cerevisiae.

Ishihara, S., Hirata, A., Minemura, M., Nogami, S. and Ohya, Y.
Mol. Genet. Genomics
2001 265: 585-595.

Yeast 1,3-β-Glucan Synthase Activity Is Inhibited by Phytosphingosine Localized to the Endoplasmic Reticulum.

Abe, M., Nishida, I., Minemura, M., Qadota, H., Seyama, Y., Watanabe, T. and Ohya, Y.
J. Biol. Chem.
2001 276: 26923-26930.

Yeast Lrg1p Acts as a Specialized RhoGAP Regulating 1,3-β-Glucan Synthesis.

Watanabe, D., Abe, M. and Ohya, Y.
Yeast
2001 18: 943-951.

Complementing Yeast rho1 Mutation Groups with Distinct Functional Defects.

Saka, A., Abe, M., Okano, H., Minemura, M., Qadota, H., Utsugi, T., Mino, A., Tanaka, K., Takai, Y. and Ohya, Y.
J. Biol. Chem.
2001 276: 46165-46171.

Appearance of poor-fermenting variants in brewing yeast culture.

Yamagishi, H., Yuuki, T., Kagami, N., Yokogawa, Y., Shibata, K., Minemura, M. and Ohya, Y.
Biosci. Biotechnol. Biochem.
2001 65: 2361-2363.

2000年

A fluorescent indicator for detecting protein-protein interactions in vivo based on protein splicing.

Ozawa T., Nogami, S., Sato, M., Ohya, Y. and Umezawa, Y.
Anal. Chem.
2000 72: 5151-5157.

1999年

An FH domain-containing Bnr1p is a multifunctional protein interacting with a variety of cytoskeletal proteins in Saccharomyces cerevisiae.

Kikyo, M., Tanaka, K., Kamei, T., Ozaki, K., Fujiwara, T., Inoue, E., Takita,Y., OhyaY. and Takai, Y.
Oncogene
1999 18: 7046-7054.

Complementation analysis in PtdInsP kinase-deficient yeast mutants demonstrates that Schizosaccharomyces pombe and murine Fab1p homologues are phosphatidylinositol 3-phosphate 5-kinases.

McEwen, R. K., Dove, S. K., Cooke, F. T., Painter, G. F., Holmes, A. B., Shisheva, A., Ohya, Y., Parker, P. J. and Michell, R. H.
J. Biol. Chem.
1999 274: 33905-33912.

Prenylation of Rho1p is required for activation of yeast 1,3-β-glucan synthase.

Inoue, S. B., Qadota, H., Arisawa, M., Watanabe, T. and Ohya, Y.
J. Biol. Chem.
1999 274: 38119-38124.

Recognition and cleavage of double-stranded DNA by yeast VMA1-derived endonuclease.

Miyamoto, S., Mizutani, R., Satow, Y., Kawasaki, M., Ohya, Y. and Anraku, Y.
Nucleic Acids Symp. Ser.
1999 42: 197-198.

1998年

Phosphatidylinositol-4-Phosphate 5-Kinase Localized on the Plasma Membrane Is Essential for Yeast Cell Morphogenesis.

Homma, K., Terui, S., Minemura, M., Qadota, H., Anraku, Y., Kanaho, Y. and Ohya, Y.
J. Biol. Chem.
1998 273: 15779-15786.

Amino Acid Residues That Define Both the Isoprenoid and CAAX Preferences of the Saccharomyces cerevisiae Protein Farnesyltransferase. Creating the perfect farnesyltransferase.

Caplin B. E., Ohya, Y. and Marshall, M. S.
J. Biol. Chem.
1998 273: 9472-9479.

Identification of Functional Connections between Calmodulin and the Yeast Actin Cytoskeleton.

Sekiya-K., M., Botstein, D. and Ohya, Y.
Genetics
1998 150: 43-58.

Importance of Phenylalanine Residues of Yeast Calmodulin for Target Binding and Activation.

Okano, H., Cyert, M. and Ohya, Y.
J. Biol. Chem.
1998 273: 26375-26382.

Helliwell S. B., Schmidt, A., Ohya Y. and Hall M. N.

The Rho1 effector Pkc1, but not Bni1, mediates signalling from Tor2 to the actin cytoskeleton.
Curr Biol.
1998 5: 1211-1214.

1997年

Applications of the long and accurate polymerase chain reaction method in yeast molecular biology: Direct sequencing of the amplified DNA and its introduction into yeast.

Takita, Y., Takahara, M., Nogami, S., Anraku, Y. and Ohya, Y.
Yeast
1997 13: 763-768.

Molecular cloning and characterization of Drosophila genes encoding small GTPases of the rab and rho families.

Sasamura, T., Kobayashi, T., Kojima, S., Qadota, H., Ohya, Y., Masai, I. and Hotta, Y.
Mol. Gen. Genet.
1997 254: 486-494.

Identification of three core regions essential for protein splicing of the yeast Vma1 protozyme.

Kawasaki, M., Nogami, S., Satow, Y., Ohya, Y. and Anraku, Y.
J. Biol. Chem.
1997 272: 15668-15674.

Probing novel elements for protein splicing in the yeast Vma1 protozyme: a study of replacement mutagenesis and intragenic suppression.

Nogami, S., Satow, Y., Ohya, Y. and Anraku, Y.
Genetics
1997 147: 73-85.

Protein splicing in the yeast Vmal protozyme: Evidence for an intramolecular reaction.

Kawasaki, M., Satow, Y., Ohya, Y. and Anraku, Y.
FEBS Lett.
412: 518-520.

Cloning of the RHO1 gene from Candida albicans and its regulation of β-1,3-glucan synthesis.

Kondoh, O., Tachibana, Y., Ohya, Y., Arisawa, M. and Watanabe, T.
J. Bacteriol.
1997 179: 7734-7741.

1996年

Identification of yeast RHO1 GTPase as a regulatory subunit of the 1,3-β-glucan synthase.

Qadota, H., Python, C., Inoue, S. B., Arisawa, M., Anraku, Y., Zheng, Y., Watanabe, T., Levin, D. E. and Ohya, Y.
Science
1996 272: 279-281.

ROM7/BEM4 encodes a novel protein that interacts with the Rho1p small GTP-binding protein in Saccharomyces cerevisiae.

Hirano, H., Tanaka, K., Ozaki, K., Arisawa, M., Watanabe, T., Imamura, H., Kohno, H., Hihara, T., Kameyama, T., Hotta, K., Qadota, H., Ohya, Y. and Takai, Y.
Mol. Cell. Biol.
1996 16: 4396-4403.

Activation of yeast protein kinase C by Rho1 GTPase.

Kamada, Y., Qadota, H., Python, C. P., Anraku, Y., Zheng, Y., Ohya, Y. and Levin, D. E.
J. Biol. Chem.
1996 271: 9193-9196.

Signaling toward yeast 1,3-β-glucan synthesis.

Inoue SB, Qadota H, Arisawa M, Anraku Y, Watanabe T, Ohya Y.
Cell Struct. Funct.
1996 21: 395-402. Review.

Bni1p implicated in cytoskeletal control is a putative target of Rho1p small GTP-binding protein in Saccharomyces cerevisiae.

Kohno, H., Tanaka, K., Mino, A., Imamura, H., Fujiwara, T., Hotta, K., Qadota, H., Watanabe, T., Ohya, Y. and Takai, Y.
EMBO J.
1996 15: 6060-6068.

Folding-dependent in vitro protein splicing of the Saccharomyces cerevisiae VMA1 protozyme.

Kawasaki, M., Makino, S., Matsuzawa, H., Satow, Y., Ohya, Y. and Anraku, Y.
Biochem. Biophys. Res. Commun.
1996 222: 827-832.

Mutational analysis of the β-subunit of yeast geranylgeranyl transferase I.

Ohya, Y., Caplin, B. E., Qadota, H., Tibbetts, M. F., Anraku, Y., Pringle, J. R. and Marshall, M. S.
Mol. Gen. Genet.
1996 252: 1-10.

LA-PCR-based quick method for the identification of genes responsible for the complementation of Saccharomyces cerevisiae mutations.

Ohya, Y.
BioTechniques
1996 20: 772-778.

Augmentation by calmodulin of ADP-ribosylation factor-stimulated phospholipase D activity in permeabilized rabbit peritoneal neutrophils.

Takahashi, K., Tago, K., Okano, H., Ohya, Y., Katada, T. and Kanaho, Y.
J. Immunol.
1996 156: 1229-1234.

Yeast Cls2p/Csg2p localized on the endoplasmic reticulum membrane regulates a non-exchangeable intracellular Ca²⁺ pool cooperatively with calcineurin.

Tanida, I., Takita, Y., Hasegawa, A., Ohya, Y. and Anraku, Y.
FEBS Lett.
1996 379: 38-42.

総説・その他